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Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat: A freeze-fracture study Biocell
Cavicchia,Juan Carlos; Guembe,Gustavo; Fóscolo,Mabel.
In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe 'en face' the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Changes in nuclear pores during pregnancy begin with an intense reduction in number but still showing an even distribution on the nuclear membrane, never forming aggregations sharply separated from pore-free...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apoptosis; Corpus luteum; Nuclear envelope; Nucleocytoplasmic transport.
Ano: 2010 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452010000200004
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The actin filament network associated to Sertoli cell ectoplasmic specializations Biocell
Cavicchia,Juan Carlos; Fóscolo,Mabel; Ibañez,Jorge; Lillig,Christopher; Capani,Francisco.
Junctional devices in Sertoli cells conform the blood-testis barrier and play a key role in maturation and differentiation of germ cells. The spacial distribution of ectoplasmic specializations of Sertoli cells was studied by β-actin immunolabelling, using laser confocal and transmission electron microscopy. For confocal microscopy, β-actin immunolabelling of ectoplasmic specializations was studied over the background of either prosaposin or glutaredoxin immunolabelling of the Sertoli cytoplasm. Labelling was found near the basal lamina, surrounding early spermatocytes (presumably in leptotene-zygotene) or at one of two levels in the seminiferous epithelium: (1) around deep infoldings of the Sertoli cell cytoplasm, in tubular stages...
Tipo: Info:eu-repo/semantics/article Palavras-chave: β -actin Ectoplasmic specializations Sertoli cell Seminiferous epithelium.
Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000300003
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